Establishment of Rapid Diagnosis Methods of Infectious Pancreatic Necrosis Virus

• Main Authors: Chuang, Shu-Chin, 莊舒菁
• Other Authors: Chian-Ren Jeng
• Format: Others
• Language: zh-TW
• Published: 1998
• Online Access: http://ndltd.ncl.edu.tw/handle/15576223721267300536

碩士 === 國立臺灣大學 === 獸醫學系 === 86 === Since isolated from rainbow trout in 1983 in Taiwan, the IPNV (Infectious Pancreatic Necrosis Virus) was identified in many kind of marine and fresh water fish continuously. In addition to horizontal transmission between fish, the vertical transmission via carrier and eggs causes a large number of deaths of fingerlings. Because the source of rainbow trout eggs completely depends on importation, this experiment is designed to build a rapid diagnostic protocol of IPNV to prevent spread of virus via carrier and eggs. There are two approach in this experiment: (1)RT-PCR: Utilize proteinase K to digest the virus packaged in the shell membrane to explore the viral nucleic acid, then detected using RT-PCR. (2) In situ hybridization: The probe was labelled with Digoxigenin, then applied in ' in situ hybridization'. Applied the probe to detect the early stage of traditional viral isolated cell line cultivation, and the sensitivity is 101-2 TCID50/ml. In the other hand, detected the sections of organs of the inoculated rainbow trout and eel fingerlings, and the positive infected cells in the liver, kidney, gut and skin were detected , and prove this technique can be applied for clinical diagnosis.