| Summary: | 碩士 === 國立臺灣大學 === 醫事技術學系研究所 === 86 === Abstract
Serratia marcescens shows cell differentiation phenomena and population
surface migration (swarming) on LB swarming agar plates. The underlying
mechanisms why such phenomena occur are not clear and it is postulated
that solid surface contact is an essential factor for the initiation of
cell differentiation. The acyl homoserine lactone (AHL) signals are reported
to be involved in the regulation of initiation of swarming in Serratia
liquefaciens. A series of similar AHL signals are also observed to have
significant effects on the swarming behaviour of S. marcescens. As RspA
is reported to inhibit the amount of a Vibrio fischeri quorum-sensing AHL
signal(OHHL) after it is synthesized in Escherichia coli, I hypothesized
that RspA can affect the swarming behaviour of S. marcescens through
regulation of the amount of AHL signals. A rspA gene was cloned from
S. marcescens by using polymerase chain reaction (PCR). Results showed
that while S. marcescens swarming is inhibited in-trans by multi-copy
rspASm, the amount of AHL signal synthesized is not affected. A possible
explanation for this observation is discussed.
Many groups of bacteria exhibit cooperative behavioral patterns such
as bioluminescence emission, antibiotic production, virulence factor
expression and the population surface migration (swarming). I show here
that in S. marcescens diffusible signal molecules are involved in the
initiation of swarming. Synthesis of the signals is dependent on a
functional smaI gene, the predicted translation product of which exhibits
substantial homology to the luxI family of putative homoserine lactone
synthase. These results add swarming motility to the rapidly expanding
list of phenotypes known to be controlled by quorum-sensing signal molecules.
My data showed that the initiation of swarming was controlled through
quorum sensing system and smaI was important for the process of swarming
but not for the initiation of cell differentiation.
褲年度：86
學期：2
校院：國立台灣大學
系所：醫事技術學系研究所
論文名稱(中)：以微衛星標記分析台灣地區膀胱癌患者
! 論文名稱(英)：MIcrosatellite Analysis of Bladder Cancer in Taiwan
學位類別：碩士
語文別：chi
! 學號：R85424015
提要開放使用：是
頁數：2
研究生(中)姓：鄭
研究生(中)名：靜芸
研究生(英)姓：Cheng
研究生(英)名：Ching-Yurn
Mcrosatellite (short tandem repeat) are repeatitive saquence of mostly 2 to
4 nucleotides with a widespread occurence particularly in multicellular organ
isms. In the process of tumor formation, the cell might have LOH (loss of hete
rozygosity) or genomic instability. Repeat saquence such as microsatellite sl
ip easily during DNA replication and induce microsatellite expansion or deleti
on. IF the vriable region is in the proximity of proto-oncogenes or tumor sup
pressor genes,function of these genes may be altered and induce cancer. Theref
ore, microsatellite has been used as a tool to detect human cancers, includin
g lung, bladder, liver and breast cancers. 27 patietnes with TCC (transitional
cell carcinoma) of bladder cancer were screened with eleven
microsatellite markers
that were widely used in detection of LOH a
nd genomic instability of bladder cancer.Our results that 17 out of 27 patient
s (63%) have LOH and genomic instability and the frequence of mutation is high
er on chromosome 9.
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