| Summary: | 碩士 === 國立臺灣大學 === 環境衛生研究所 === 86 === Lipid peroxidation in vivo always produces aldehydes. For
example, malondialdehyde (MDA) is produced by lipid
peroxidation. MDA is biologically active to bind with DNA or
proteins. This property may destroy the development of normal
cells and subsequently cause tumorgenesis. The compounds
containing primary amine could react with MDA to form adducts
through Schiff base reaction. MDA could also react with thiols
by the way of Michael addition.
In this study, the reactivity of MDA with some biochemical
compounds was measured by UV/Visible spectrophotometer, high
performance liquid chromatography (HPLC) and electrospray HPLC/
MS. Two condensation products, MDA-deoxyguanosine and MDA-
cysteine, were confirmed in this study. The result also
indicates that MDA reacted with both 2-deoxyguanosine and
cysteine to form several other adducts, and the structure of
the products were further analyzed by LC/MS.
Keywords : malondialdehyde, aldehyde adduct, LC/MS
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