| Summary: | 碩士 === 國立臺灣大學 === 漁業科學研究所 === 86 === In this study, the partial cDNA encoding for the sGnRH precursor,cGnRH-II
precursor and sbGnRH precursor were isolated from tilapia brain using RT-PCR
and 5''-RACE. The partial 145 bp cDNA encoding sGnRH precursor
is composed of the
biologically active sGnRH, the cleavage site (Gly-Lys-Arg), and a partial GnRH-
associated peptide (GAP). The partial 366 bp cDNA encoding cGnRH-IIprecursor is
composed of 116 bp 5''-untranslation region (5''-UTR), the signal peptide, the
biologically active cGnRH-II, the cleavage site, and a partial GAP. The partial
216 bp cDNA encoding sbGnRH precursor is composed of 69 bp 5''-UTR, the signal
peptide, the biologically active sbGnRH, the cleavage site,and a partial GAP.
In order to investigate the expression of three forms of GnRH in tilapia
ovary, total RNA from ovary of female tilapia at different ovary stages was
amplified by RT-PCR. The predicted sizes of RT-PCR products were amplified in
ovary. These results demonstrate that three forms of GnRH
precursor (sGnRH,cGnRH
-II and sbGnRH) mRNA are expressed in tilapia ovary tissue and that high level
of cGnRH-II transcript and low level sGnRH and sbGnRH transcript are expressed
in tilapia ovary.
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