| Summary: | 碩士 === 國立臺灣大學 === 農業化學系 === 86 === Purification of bioactive ingredient from pesticides could increase
activity and reduce usage amounts of pesticide. In this study, instead of
classical asymmetric synthesis, separation of enantiomers were performed
with high performance liquid chromatography (HPLC) through Whelk-O1
chiral column, daphnia will use as bioassay organism for toxicity test. In
the other hand, cholinesterase is used to detect the inhibitory ability of the
enantiomers.
In the toxicity tests of daphnia, the (+)-Fenamiphos, (-)-Fenamiphos and
(+)-Fenamiphos give LC50 values of 0.0016μg/mL, 0.0061μg/mL and
0.0019μg/mL. There is no significance difference of LC50 values between
(+)-Fenamiphos and (+)-Fenamiphos, and LC50 values of (-)-Fenamiphos
show significance different. And the inhibition tests of cholinesterase
give I50values of 0.0080μg/mL, 0.15μg/mL and 0.46μg/mL, Both of
the two enantiomers and the racemate show significance different in
inhibitory activity of cholinesterase.
In the study of degradation in soil and water sample, the half-life of (+)-
Fenamiphos,
(-)-Fenamiphos and (+)-Fenamiphos in soils are: Td soil ( 24.5,
38.83, 39. 37 days),
Tc soil ( 60.43, 38.69, 11.32 days), Pc soil ( 34.47, 192.54, 173.28 days) and
Lk soil ( 41.01, 130.78, 18.83days), respectively. And the half-life in water
sample: Moon sun lake water sample ( 57.28, 64.18, 52.51 days), Ta du river
( 51.73, 50.22, 75.34 days) and Green lake ( 29, 50.59, 106.6 days),
respectively.
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