Isolation of a specific antigen of Helicobacter pylori-associated with mucosa-associated lymphoid tissue lymphoma of the stomach
碩士 === 國立臺灣大學 === 微生物學研究所 === 86 === To identify the putative specific antigens from Helicobacter pylori that isiso ated from gastric specimens of the mucosa-associated lymphoid tissue lymphoma MALToma), secreted proteins of H. pylori were compared on SDS...
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| Other Authors: | |
| Format: | Others |
| Language: | zh-TW |
| Published: |
1998
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| Online Access: | http://ndltd.ncl.edu.tw/handle/75360965524417741502 |
| Summary: | 碩士 === 國立臺灣大學 === 微生物學研究所 === 86 === To identify the putative specific antigens from Helicobacter pylori that isiso
ated from gastric specimens of the mucosa-associated lymphoid tissue lymphoma
MALToma), secreted proteins of H. pylori were compared on SDS-PAGE by Westernb
lot using sera from patients with MALToma. A 19kDa. protein was seen in strain
sisolated from patients with MALToma but not in strains from those with other
diseases.The protein was purified and amino acid sequences were determined by
microsequencing.Sequences comparision revealed it was a FldA homologue. DNA se
quencing in sixteen bacterial strains revealed that insertion of a nucleotide
"G" at position 481 ofthe fldA gene were more frequently observed in strains
associated with MALToma than those with other diseases (8/8 vs 2/8, p<0.01). T
he fldA gene from a strain associated with MALToma was cloned and recombinant
protein was expressed in E. coli.Serum samples of 12 out of 17 (70.6%) patient
s with MALToma were positive for theantibody to the recombinant FldA protein w
hile 7 out of 42 (16.7%) patients with other diseases were positive (12/17 vs
7/42, p<0.0001). These results suggest that this antigen of H.pylori may be as
sociated with gastric MALToma. It may playa role in the pathogenesis of gastri
c MALToma. Antibody to this antigen may be a serologic marker of the disease.
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