| Summary: | 碩士 === 國立臺灣大學 === 園藝學系研究所 === 86 === Morphometry of spore germination and gametophyte development in Adiantum capil
lus-veneris and Nephrolepis auriculata were measured by using steromicroscope
and dissecting microscope. Most of the spores were observed to germinate withi
n the same annulus. In A. capillus-veneris, the antheridia mixed with archegon
ia on the cushion were seen in vivo; gametophytes while only antheridia were p
roduced from in vitro. There were numerous single-celled trichome on the margi
n and both surfaces of the gametophytes in Nauriculata. Both in vivo and in vi
tro culture, the sporophytic fronds were developed from the underside of the g
ametophytes in A. capillus-veneris and N. auriculata.
The greatest spore germination of A. capillus-veneris was recorded to be 8
2.5% in MS medium. A rapid and mass increase in gametophyte was produced in MS
medium supplemented with 2% sucrose. Gametophytes were not browning with the
addition of activated charcoal after 6 months'' subculture. It required 50 days
after in vitro transfer of gametophytes to spororphtic frond visibility in so
illess. This shortened the time from spore sowing to sporophyte visibility by
38 days, as compared to direct sowing in the soiess medium. Ef
fects of temperature and mineral nutrition were also studied on growth of N. a
uriculata, A. capillus-veneris and Adiantum raddianum cv. Fritz-Luethii grown
in phytotron and natural greenhouse condition. The greatest gametophyte and sp
orophyte growth in N. auriculata and A. capillus-veneris were at day/night tem
perature of 25/20℃. Plants of A. raddianum cv. Fritz-Luethii treated at 25/20
℃ also produced more frond numbers, number of frond with sori and frond area.
A full strength of Johnson''s solution proced good growth of A. raddianum cv.
Fritz-Luethii, as compared with quarter to a half strength for N. auriculata.
The frond area of
the N. auriculata and A. capillus-veneris increased as the light level increas
ed from 0.6 to 5.9MJ/m2d. The optimum shadings were 19% for N. auriculata, 19-
53% for A. capillus-veneris and 61-81% for A. raddianum cv. Fritz-Luethii. A.
raddianum cv. Fritz-Luethii produced from 61 to 81% shadings resulted in bette
r interior performance quality.
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