Tissue culture and in vitro flowering of Bambusa edulis

• Main Authors: Lin, Choun-sea, 林崇熙
• Other Authors: Chang, Wei-chin
• Format: Others
• Language: zh-TW
• Published: 1998
• Online Access: http://ndltd.ncl.edu.tw/handle/40635362349934044312

博士 === 國立臺灣大學 === 園藝學系研究所 === 86 === Nodal explants obtained form 10 year-old field-growth culms of Bambusa edulis produced mutiple shoots on a Murashige and Skoog (1962) medium supplemented w ith 0.1 mg/l thidiazuron (TDZ). Multiple shoots subcultured in MS (Murashige and Skoog, 1962) medium supplemented 0.1 mg/l TDZ flor 3-10 months formed spik elets. Sections of spikelets and multiple shoots were subcultured in MS medium containing cytokinins (2 mg/l kinetin or 0.01-0.1 mg/l TDZ),1-5 mg/l 2,4-dich lorophenoxyacetic acid (2,4-D) for callus initiationand somatic embryogenesis. Somatic embryo germinated on MS based medium supplemented with 0.1 mg/l TDZ a nd eventually proliferated tomultiple shoots. Multiple shoots flowered in same medium. All themultiple shoots derived from either shoot buds or somatic embr yos were capable to flower after subcultures. Spikelets produced more sipkelet s when subcultured in same medium. Also albino spilelets were able to prolifer ate in same medium. This spikelet-produced-spikelet system had been maintained for 2.5 years. Flowering in vitro and in vivo occurred in rooted explants.