Summary: | 博士 === 國立臺灣大學 === 園藝學系研究所 === 86 === Nodal explants obtained form 10 year-old field-growth culms of Bambusa edulis
produced mutiple shoots on a Murashige and Skoog (1962) medium supplemented w
ith 0.1 mg/l thidiazuron (TDZ). Multiple shoots subcultured in MS (Murashige
and Skoog, 1962) medium supplemented 0.1 mg/l TDZ flor 3-10 months formed spik
elets. Sections of spikelets and multiple shoots were subcultured in MS medium
containing cytokinins (2 mg/l kinetin or 0.01-0.1 mg/l TDZ),1-5 mg/l 2,4-dich
lorophenoxyacetic acid (2,4-D) for callus initiationand somatic embryogenesis.
Somatic embryo germinated on MS based medium supplemented with 0.1 mg/l TDZ a
nd eventually proliferated tomultiple shoots. Multiple shoots flowered in same
medium. All themultiple shoots derived from either shoot buds or somatic embr
yos were capable to flower after subcultures. Spikelets produced more sipkelet
s when subcultured in same medium. Also albino spilelets were able to prolifer
ate in same medium. This spikelet-produced-spikelet system had been maintained
for 2.5 years. Flowering in vitro and in vivo occurred in rooted explants.
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