| Summary: | 碩士 === 國立臺灣大學 === 植物學系研究所 === 86 === Nicotiana plumbaginifolia has 10 pairs of acrocentric or telocentricchromosome
s, which could not be distinguished from each other by conventional cytologica
l techniques. The somatic karyotype of this species was constructed based on f
luorescence in situ hybridization (FISH) to a 165 bp tandemly repeated sequenc
e, NP3R. Variation in the spatial and quantitative distribution of this repeat
allowed the identification of each of the 10 pairs of N. plumbaginifolia chr
omosomes. Two-step FISH and multi-color FISH, showed that 5S rDNA was clustere
d in a proximal region in the long arm of chromosome 2, and that 26S rDNA was
clustered in the terminal regions of chromosomes 9 and 10.
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