Cloning and characterization of maize beta-amylase gene

• Main Authors: Lin, Yi-Nan, 林以楠
• Other Authors: Shue-Mei Wang
• Format: Others
• Language: zh-TW
• Published: 1998
• Online Access: http://ndltd.ncl.edu.tw/handle/42569849646669298050

碩士 === 國立臺灣大學 === 植物學系研究所 === 86 === Bta-amylase is one of the prevalent starch degrading enzymes in plants. It ex hibits an exo-amylolytic activity and releases beta-maltose molecules from sta rch in vitro. In germinating maize kernels, however, beta-amylase is expresse d in the aleurone cells, which contain no starch for its exo-amylolytic action . Difference in the localization of enzyme and substrate molecules makes it d ifficult to understand the physiologicalfunctions of beta-amylasein vivo. To further investigate its functions, maize genomic libraries were constructed, a nd a full-length beta-amylase gene was cloned and sequenced. The primary stru cture of beta-amylase was also deduced from the sequence data. Beta-amylase p romoteractivity analysis was also undertaken with transient expression systems . Three different systems were tested, but proved unsuccessful. The promoter sequences were also analyzed by searching through internet resources and rese arch articles. Possible cis-elements in promoter region were found by databas e searching. Moderate degrees of homology were also found by computer-aided a lignment around TATA box and 5''-UTR region among beta-amylase promoters of mai ze, barley, and rice. These results could provide insights into related resea rch and data interpretation on beta-amylase in the future.