Renal Response to Acute Unilateral Ureteral Occlusion in Rats

• Main Authors: Yeh, S-Y, 葉思妤
• Other Authors: Chien, C.-T.
• Format: Others
• Language: zh-TW
• Published: 1998
• Online Access: http://ndltd.ncl.edu.tw/handle/13688521445263630390

碩士 === 國立臺灣大學 === 生理學研究所 === 86 === Urinary tract obstruction is an important cause of renal insufficiency. Unilat eral ureteral obstruction (UUO) is a useful model to study the renal response to urinary tract obstruction. The acute phase of renal response to UUO was stu died in female rats weighing 190-236 g. We recorded the ureteral pressure and changes of renal parameters all the way during a 120 minutes of UUO in control (US, UUO+saline), L-arginine treated (UA), right nephrectomized rats (UO, UUO in one kidney) and other 3 sham control groups. After complete ureteral obstr uction, ureteral pressure increased in all 3 groups of rats, the extent of inc rease was not significantly different in US and UA rats, but significantly hig her in UO rats. It was found that in US rats, their cortical microvascular blo od flow (CMVBF), measured by Laser Doppler Flowmeter, declined significantly f rom 321±10 perfusion unit (PU) to 260±11 PU. Percent drop in CMVBF at 120 mi n of UUO was significantly augmented in UO (25.7±3.8 %) than US (19±2.1 %) a nd UA (14±2%). Acute UUO reduced GFR in US and UO rats, however, administrati on of L-arginine attenuated the GFR decrease. The excretion of nitrate/nitrite was increased after UUO. Western blot of endothelial nitric oxide synthase (e NOS) in renal cortex and medulla protein extracts from US and shammed operated rats were no significant difference in their expression. Finally, this experi ment showed that in the early phase of renal response to UUO, there were no hy peremia in rats with two or one kidney(s). L-arginine attenuated the renal res ponse to UUO. Involvement of NO in the response after UUO still cannot be conc luded, and further study will be necessary.