Investigation of the Correlation Between Human Papillomaviruses and Cervical Cancers in Taiwan by in Situ Polymerase Chain Reaction

• Main Author: 邱家昌
• Other Authors: 韓鴻志
• Format: Others
• Language: zh-TW
• Published: 1998
• Online Access: http://ndltd.ncl.edu.tw/handle/65768265401776198868

碩士 === 國防醫學院 === 病理及寄生蟲學研究所 === 86 === 　　Approximately half a million new cases of cervical cancer are diagnosed worldwide each year. This malignancy is now the lead in cancer incidence and is the fifth leading cause of cancer deaths among Taiwan. The etiologic association between human papillomaviruses (HPVs)and cervical cancers is now well established. Among 80 kinds of HPV discovered until now, about 36 HPV subtypes are involved in anogenital infection and HPV16/18 are known to be the representative highly oncogenic viral types of cervical cancer. In situ polymerase chain reaction (ISPCR), a new method that combines the high sensitivity of the polymerase chain reaction (PCR) with the cytological and localization of sequences provided by in situ hybridization (ISH).We examined 85 cervical specimens from Tri-Services General Hospital, including 22 carcinoma in situ(CIS),45 squamous cell carcinoma(SQ),3 samll cell carcinoma(SM), 12 adenocarcinoma(AD) and 3 adenosquamous cell carcinoma (AS) by in situ hybridization (ISH) and digoxigene-11 dUTP label in situ polymerase chain reaction(ISPCR), to evaluate the association between HPV and cervical cancer in Taiwan. 　　Results:(1)Detected by ISH and IS-PCR, the HPV infection rates were 55.6%(10/18) and 88.9%(16/18). (2)Detected by direct IS-PCR, the HPV, HPV-16 and HPV-18 infection rates were 85.9%(73/85), 62.4%(53/85) and 24.7%(21/85), (3)According to clinical stage, the HPV infection rates were stage 0:90%(20/22), stage Ⅰ:87.5%(21/24), stageⅡ:78.9%(15/19), stage Ⅲ: 80%(4/5), and stage Ⅳ:71%(5/7); HPV16 infection rates were 68.2%(15/22), 54.2%(13/24), 52.6%(10/19), 40.0%(2/5), 42.9%(3/7)；HPV18 infection rates were 27.3%(6/22), 16.7%(4/24), 15.8%(3/19), 60.0%(3/5), 28.6%(2/7).(4)According to the histopathologic type, the infection rates of HPV were SQ:86.7%(39/45), AD:69.2%(9/13), AS:100.0%(2/2), SM: 100.0%(3/3); HPV16 infection rates were 66.7%(30/45), 7.7%(1/13), 100.0%(2/2), 66.7%(2/3); HPV18 infection rates were 11.1%(5/45), 46.2%(6/13), 0%(0/2), 66.7%(2/3). 　　Conclusion: (1)ISPCR(16/18) is a valuable and sensitive method than ISH (10/18) for detection of HPV. And after in situ amplification, HPV DNA were also detected in basal, parabasal cells and tumor cells that were not detected by ISH. (2) HPV16 was predominant in squamous cell carcinoma (66.7%; 30/45)(P=0.002) and HPV18 was predominant in adenocarcinoma (46.2%; 6/13) (P=0.014). (3)HPV18 was associated with high stage (stageⅢ+Ⅳ ) of cervical cancer (P=0.034). (4)There were no significant correlation between HPV status and patients'' age.