| Summary: | 碩士 === 國防醫學院 === 生物及解剖學研究所 === 86 === Parkinson''s disease is a neurodegenerative disorder due to the loss of dopaminergic neurons in the substantia nigra that project to striatal neurons. The decrease of dopamine in striatum resuslts in unique motor and cognitive deficits. Neurotransplantation of fetal substantia nigral tissue is shown to improve clinical symptoms and recognized as a treatment of choice. However, immunological rejection, source and survival of nigral tissue are the major problems which hamper the neurotransplantation. Since Sertoli cells express a variety of immunosuppressive and trophic factors, Sanberg and his colleagues tried to apply Sertoli cells in neurotransplantation for Parkinson''s disease. This thesis inxestigagted immunosuppresive and neurotrophic effects of Sertoli cells transplanted in striatum of parkinsonian rats. Dopaminergic neurons in sustantia nigra of male S-D rats were lesioned by 6-hydroxydopamine (6-OHDA) stereotaxically injected into right medial forebrain bundle. Damage of dopaminergic neurons (≧90%) was confirmed by increased amphetamine-inxtuced ipsilateral. rotation, and decreased immunoreactivity of tyrosine hydroxylase (TH) in ipsilateral striatum. Amphetamine-induced rotation was attenuated and immunoreactivity of TH in striatum was elevated after intrastriatal transplantation of Sertoli cells. In addition, Sertoli cells expressed P-cadherin were found in the striatun of the rats 14 weeks after transplantation without cyclosporine A. These results indicated that Sertoli cells transplanted in striatum of parkinsonian rats exhibited immunosuppresive and neurotrophic effects.
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