Determination of Organophosphate and Carbamate pesticides by Acetylcholinesterase inhibition using Sequential Injection Analysis

• Main Authors: Chung- Shu-Wen, 張舒雯
• Other Authors: Shu Hun-Chi
• Format: Others
• Language: zh-TW
• Published: 1998
• Online Access: http://ndltd.ncl.edu.tw/handle/82863138397600604899

碩士 === 國立東華大學 === 化學研究所 === 86 === Organophosphate and carbamate pesticides such as paraoxon, dichlorovos and carbofuran were analyzed by automatic sequential injection analysis (SIA). The analytical system is fully computerized and is able to monitor the concentration of the pesticide residues in an assay cycle of 180 s including 30 s incubation time when the flow is stopped. The optimal operation conditions such as enzyme, substrate, pH, flow rate and stopping time were studied. The experimental set-up gave good reproducibility with the linear ranges for paraoxon (1-70 ppb), dichlorovos (20-70 ppb), and carbofuran (2-70 ppb). The detection limit of paraoxon, dichlorovos and carbofuran were 1, 5 and 5 ppb, respectively. It is a new application for SIA system to determine the trace concentration of organophosphate and carbamate pesticides based on the inhibition of acetylcholinesterase.