Triblock copolymers as displacers in anion-exchange displacement chromatography for proteins separation

• Main Authors: Tai, Kuo-Che, 戴國哲
• Other Authors: Chen Wen-Yih
• Format: Others
• Language: zh-TW
• Published: 1998
• Online Access: http://ndltd.ncl.edu.tw/handle/64669429474259233858

碩士 === 國立中央大學 === 化學工程學系 === 86 === Many successful studies have underscored the potential of displacement chromatography as an economic high-throughput preparative technique. Although the ability to carry out simultaneous concentration and purification in single displacement step has significant advantages for downstream processing of biopharmaceuticals, a major impediment to the implementation of displacement chromatography has been the lack of suitable displacer compounds. To tackle this problem, a series of acrylic triblock copolymer were synthesized. Methacrylic block copolymer as one of the newest polymers shows its significant solution properties, when the polymer is built with difference in composition, size and sequence of blocks. In this study, triblock copolymers were utilized in an anion- exchange displacement chromatography for the separation of a crude mixture of ?lactoglobulins into the A and B isoforms. A chromatography model utilizing steric mass action(SMA) ion- exchange formalism is employed to predict the displacement behavior of proteins. An operating regime plot is developed from the SMA model to predict displacement chromatography. We found that the higher ratio of the steric factor(? to the characteristic charge(v) of the displacer, the earlier displacement is achieved. The ?v ratio can be controlled by the variation of block size and pH value. As pH value is higher than the isoelectric point of the triblock copolymers, and the longer the A block of the copolymers, the higher the characteristic charge is, and the B and M blocks of the copolymers is lengthened, the steric factor increases. With the above discussions, the triblock copolymers then can be successfully designed and used as anion-exchange displacers for proteins separation in an appropriate condition.