| Summary: | 碩士 === 國立交通大學 === 生物科技研究所 === 86 === Deoxyribonucleic acid (DNA) at the natural state is very stable;
its half-life is over 100,000,000 years. Therefore, how to
promote the DNA's hydrolysis rate becomes a challenge for many
biochemists. In the beginning of this thesis, we are using
macrocyclic ligand 1,7-bis(carboxymethyl)
1,4,7,10-tetraazacyclododecane (DO2A) and acid-1,7-
diaza-4,10,13-trioxacyclopentadecane N,N'-Diacetic (K21DA) and
lanthanide ions La(III) and Eu(III) to form complexes, and
cleavage the phosphate diester(BNP-). By using diffFor the
cleavage of the deoxyribonucleic acid, we used the HPLC to
observe the hydrolysis of single-strand DNA primer by EuDO2A.
After 5 hours, the 26 base DNA primer was completely hydrolyzed.
We also used the agarose gel electrophoresis to observe the
result of hydrolysis of double-strand DNA.It was found that DNA
Form I (supercoiled form) decreased as the concentration of the
complex increased, and DNA Form II (circular form) increased as
the concentration of the complex increased. This proves that the
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