Studies on the Determination of Five Sulfonamide Residues in the Slaughtered-pig Urine

• Main Authors: Shih, Wei-Chung, 施偉中
• Other Authors: Tzeng Chiou-Lung
• Format: Others
• Language: zh-TW
• Published: 1998
• Online Access: http://ndltd.ncl.edu.tw/handle/26485705902660018210

碩士 === 國立中興大學 === 獸醫微生物學研究所 === 86 === The first purpose of this investigation was developing a thin-layerchromatograghic (TLC) method for applying mixed standard solution contain-ing sulfamethazine (SMT), sulfadimethoxine (SDM), sulfamonomethoxine (SMM), sulfaquinoxaline (SQX) and sulfathiazole (STZ).Plate was developed 10 cm in chloroform-n-BuOH(80:20) at 30 C water bath, then the same plate was developed 17 cm at room temperature, which were obtained the good seperationeffects. The limit detection concentration of TLC method was 1~3 ug/mL. The second purpose of this investigation was to develop a high- perform- ance liquid chromatographic (HPLC) method for the simultaneous determinationof SMT, SDM, SMM, SQX and STZ in the slaughtered- pig urine. The separation of HPLC method was carried out on C18 column (Cosmosil 5C-18-AR column;4.6 x 150 mm I.D.)with acetic acid buffer solution (pH 3.6)-acetonitrile (85:15) as the mobile phase at a flow rate of 1.0 mL/min. Thechemicals were detected by UV detector which the wavelength was set at 267nm. The samples were prepared by extraction with 1 N sulphuric acid and chloro- form twice, then were treated with solid phase dispersion for cleanup. The recovery rates of the chemicals from the slaughtered-pig urine were fortifiedat the level of 0.02~0.5 ug/mL which were 29.3~85.5 % and 63.5~77.4 % by reversal and normal cleanup. The last purpose of this investigation was to research the quality of ELISAmethod. The limit detection concentration of ELISA method was 0.001 ug/mL forSMT and SDM, The cross-reactivity of the other four sulfonamids on ELISA methodwere 0.1~2.9 %.