| Summary: | 碩士 === 國立中興大學 === 獸醫微生物學研究所 === 86 === The first purpose of this investigation was developing a
thin-layerchromatograghic (TLC) method for applying mixed
standard solution contain-ing sulfamethazine (SMT),
sulfadimethoxine (SDM), sulfamonomethoxine (SMM),
sulfaquinoxaline (SQX) and sulfathiazole (STZ).Plate was
developed 10 cm in chloroform-n-BuOH(80:20) at 30 C water bath,
then the same plate was developed 17 cm at room temperature,
which were obtained the good seperationeffects. The limit
detection concentration of TLC method was 1~3 ug/mL. The second
purpose of this investigation was to develop a high- perform-
ance liquid chromatographic (HPLC) method for the simultaneous
determinationof SMT, SDM, SMM, SQX and STZ in the slaughtered-
pig urine. The separation of HPLC method was carried out on C18
column (Cosmosil 5C-18-AR column;4.6 x 150 mm I.D.)with acetic
acid buffer solution (pH 3.6)-acetonitrile (85:15) as the mobile
phase at a flow rate of 1.0 mL/min. Thechemicals were detected
by UV detector which the wavelength was set at 267nm. The
samples were prepared by extraction with 1 N sulphuric acid and
chloro- form twice, then were treated with solid phase
dispersion for cleanup. The recovery rates of the chemicals from
the slaughtered-pig urine were fortifiedat the level of 0.02~0.5
ug/mL which were 29.3~85.5 % and 63.5~77.4 % by reversal and
normal cleanup. The last purpose of this investigation was to
research the quality of ELISAmethod. The limit detection
concentration of ELISA method was 0.001 ug/mL forSMT and SDM,
The cross-reactivity of the other four sulfonamids on ELISA
methodwere 0.1~2.9 %.
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