Development on Simultaneous Determination of eight Quinolone in Pork, Chicken and Fish by High Performance Liquid Chromatography

• Main Authors: Gong, Peir-Shen, 龔培森
• Other Authors: Wang Way-Shyan
• Format: Others
• Language: zh-TW
• Published: 1998
• Online Access: http://ndltd.ncl.edu.tw/handle/11531641943891007362

碩士 === 國立中興大學 === 獸醫學系 === 86 === The aim of this investigation is to develop a simple, rapid, and reliablehigh-performance liquid chromatographic (HPLC) method for the simultaneous determination of the first generation of quinolones (oxolinic acid, nalidixicacid, and flumequine) and the second generation of quinolones (ciprofloxacin,danofloxacin, enrofloxacin, ofloxacin, and sarafloxacin) in fish, pork, and chicken meat. The HPLC separation was carried out on a C18 column ( Cosmosil 5C18-MS column; 150mm x 4.6 mm I.D.) with a guard column with 0.1% triethylamine buffer(pH 3.0) containing 7 mM sodium dodecyl sulfate (SDS)-acetonitrile (65:35) asthe mobilephase at a flow rate of 0.7 ml/min. The chemicals were detected byUV detection ( UV wavelength programming) which wavelength was first set at 254 nm then it was changed to 282 nm immediately by a programming technique for 11 min. At the same time, the fluorescence detection was used (excitation wavelength 325nm, emission wavelength 365 nm, (excitation wavelength 280 nm, emission wavelength 450 nm). The samples were prepared by extraction with acetonitrile and methanolicsodium hydroxide solution flowed by liquid-liquid partition with acetonitrile saturated n-hexane for clean up. The recoveries of the chemicals from fish, pork,and poultry meat fortified at the level of 0.02~0.5 ug/g were 73.1~91.7%, withhigh precision. The limit of detection of our developed method was 0.01 ug/g foreach drug. Each 50 samples of fish, pork, and chicken meat from market located in Taiwan area were surveyed by random for the residues of quinolones with the developed method. The results of the residues of quinolones in chicken, pork, and fish samples were 2%, 0%, and 0%, respectivately.