| Summary: | 碩士 === 高雄醫學院 === 公共衛生學研究所 === 86 === AstractThere are MspI polymorphism and Val polymorphism in CYP1
A1 gene. There are differ distribution of many ethnic groups in
the world In both polymorphisms. They were also associated with
medicine metabolism and susceptibility to neoplasm, for instance
lung cancer. In the ethnic distribution of genotype study,
subjects are junior high school students of aborigines contained
Paunch (n=169), Puyumar (n=38), Bunun (n=72), Atayal(n=72),
Paiwan(n=48), min-nan(n=57) and Philippines(n=120). In lung
cancer susceptibility and genotype study, subjects are woman
lung cancer patients without smoking(n=31) and control(n=61).
Genomic DNA were extracted from blood samples from subjects. The
target DNA fragment: were amplified by PCR, and genotype were
determined by RFLP. The subjects of previous two study were
interviewed by structure questionnaire, to understand another
environmental factors. The result of two study, the genotype
distribution of aborigines, case and control were conformed by
Hardy-Weinberg equilibrium. In Val polymorphism, the proportion
of variant genotype (Val/Val) is Philippines 2.52%, Paunch 3.3%,
Paiwan 4.9%, Atayal 6.3%, Bunun 8.1%, Puyumar 11.4%, min-nan
16.2%. There are difference of Val polymorphism among
Philippines, Paunch, Paiwan and min-nan. In MspI polymorphism,
the proportion of variant genotype (m2/m2) is Puyumar 8.8%,
Paiwan 11.1%, Philippines 11.3%, Paunch 12.6%, Atayal 16.4%,
Bunun 21.7%, min-nan 24.5%. There are difference of MspI
polymorphism among Puyumar, Philippines, Paunch and min-nan. In
another study, the odds ratio of m2/m2 and m1/m2 versus m1/m1
genotype for lung cancer is 1.05 (0.37-2.95), and odds ratio
which adjusted by demographic variable is 0.91 (0.24-3.49 ). The
odds ratio of Val/Val and Ile/Val versus Ile/Ile genotype for
lung cancer is 1.06 (0.37-3.5) , and odds ratio which adjusted
by demographic variable is 1.41(0.40-4.95). The result doesn''t
show statistic significant. Adenocarcinoma of case who
categorized by histological type still doesn''t show statistic
significant between genotype and Adenocarcinoma. The limitation
of study is less samples. Furthermore, the future of study could
research mutiple-gene and individual susceptible for lung
cancer, and interaction between environment an gene. Key words：
CYP1A1 polymorphism , aborigines , lung cancer
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