Development of High Performance Liquid Chromatography/Fourier Transform Infrared Spectrometry Interface Based on Filtering Type Solvent Elimination Technique

• Main Authors: Kao Hung Yu, 高弘豫
• Other Authors: Jyisy Yang
• Format: Others
• Language: zh-TW
• Published: 1998
• Online Access: http://ndltd.ncl.edu.tw/handle/38904275425522056799

碩士 === 中原大學 === 化學學系 === 86 === In this study, a novel interface is proposed for combing high performance liquid chromatography ( HPLC ) and Fourier Transform Infrared ( FT-IR )spectrometry. Currently, nebulization type interface is most acceptable in combination of HPLC and FT-IR. Because this type of interface needs thermal energy to assist the vaporization of the mobile phase of HPLC, problems such asclogging of transfer line and limiting to low flow rate applications are observer. After theoretically consideration of vaporization of mobile phase of HPLC (result show in Chapter 2 ), the heating type interface can not eliminate the problems mentioned above. With this proposed new type of interface, above problems can remove completely. The concept in concept in construction of this proposed interface is based upon the process of filtering. A filter is plased in theend of the transfer line to concentrate the soult while remove the mobile phase of the HPLC. the left soult are examined by FT-IT spectrometry to obtainthe IR spectra. In order to concentrate the soult into one compact spot, postiveand negative pressure are tested in their assistance of removing of mobile phase. Results indicated that by input postive pressure to the filter can obtainmuch better performance than negative type. Factors influence the concentrationof the solute on filter are also studied, such as filter materials, amount of analytes, buffer solution, variation of gradients of mobile phase and flow rate of HPLC.