Bioavailability Studies of Cephradine

• Main Author: 洪瑞泰
• Other Authors: 劉正雄
• Format: Others
• Language: zh-TW
• Published: 1998
• Online Access: http://ndltd.ncl.edu.tw/handle/64419040115921620639

碩士 === 中國醫藥學院 === 藥物化學研究所 === 86 === 　　Cephradine is a cephalosporin antibiotics active against a broad spectrum of gram-positive and gram-negative bacteria by inhibiting the synthesis of bacterial cell wall. Cephradine is the only cephalosprine that can be administrated via oral and injection. Cephradine is stable in acid solution, well absorbed via oral and excreted unchanged in the urine. 　　Two quantitative methods are adopted in this study-bioassay and HPLC to quantify the concentration of cephradine in plasma for the 12 subjects in a bioequivalency test after dosing cephradine preparations-Velosef and Sephros capsules.The results of blood samples quantified by two said analysis methods reveal no difference in the two quantitative methods by pair-t test. From the mean pharmacokinetic parameters of each subject, we found the mean serum half-life is around 0.78 hour, clearance is around 20L/hr, distributive volume is 35.5L. No variety among the pharmacokinetic parameters for each subject after statistical by pair-t test. Besides, no variety among the pharmacokinectic parameters treatment with ANOVA procedure. The result of the 90% confidence intervals by two-one sided test design is between 0.8-1.2, it reveals that Velosef an Sephros are bioequivalent. 　　Two guantitative method used in this bioequivalent and bioavailability study are validated to bear acceptable precision and accuracy. 　　From the results of this study, we can find there is some difference of cephradine pharmacokinetic characters between Chinese and foreigners, that is the clearance rate, distribution volume of cephradine is higher in Chinese than in foreigner.