Studies on the Alkaloids and Triterpenoids of Fissistigma oldhamii (Hemsl.) Merr. and Flavonoids of Moghania macrophylla (Willd.) O. Kuntze.

• Main Authors: Cheng,Yih-Dih, 鄭奕帝
• Other Authors: Kuo, Sheng-Chu
• Format: Others
• Language: zh-TW
• Published: 1998
• Online Access: http://ndltd.ncl.edu.tw/handle/40410459271287352139

博士 === 中國醫藥學院 === 藥物化學研究所 === 86 === In a preliminary bioassay, the methanolic extracts of stems and leaves of Fissistigma oldhamii, and roots of Moghania macrophylla showed significant antiplatelet aggregation, antiinflammatory and antiallergic activities. Thirteen compounds, including three aristolactams, aristolactam BII (1), aristolactam FI (2) and piperolactam C (3), three aporphines, calycinine (4), dicentrine (5) and xylopine (6), three oxoaporphines, lanuginosine (7), O-methylmoschatoline (8) and oxocrebanine (9), one 4,5-dioxoaporphine, noraristolodione (l0), two morphinandienones, O-methylflavinantine (11) and O-methylflavinine (12), and one hydro-oxadiazine, fissoldhimine (13) were isolated from the stems of Fissistigma oldhamii. Six compounds, including two steroids in a mixture of b- sitosterol (16) and stigmasterol (17 ), three pentacycIic triterpenes, taraxasterol (18), taraxerol (19) and taraxerone (20) and one aporphine isocorydine (14) were isolated from the leaves of F. oldhamii. One aminoquinone, 3'',6''-diamino- 4''-methoxy-2'',5''-quinodihydrochalcone (l5) were isolated from the unripe fruits of F. oldhamii. Among them, 1, 2, 3, 5, 7, 8, 9, 10, 12, 13, 14, 15, 16, 17, 18, 19 and 20 are isolated for the first time from this plant, on the other hand, l2 and l3 are isolated for the first time from the natural sources. Thirteen compounds, including six isoflavones, genistein (21), genistin (22), ambocin (23), daidzein (24), daidzin (25) and 2''-hydrogenistin (26) , three flavanones, fleminone (27), eriosemaone A (28) and lespedezaflavanone A (29), one flavan-3-ol, ourateacatechin (30), one flavonol, moghanin A (31), and two steroids in a mixture of b-sitosterol-3-0-b-glucoside (32) and stigmasterol-3-0-b-glucoside (33) were isolated from the roots of M. macrophylla. Among them, 22, 23, 24, 25, 26, 28, 29, 30 and 3l are isolated for the first time from this plant, on the other hand, 31 is isolated for the first time from the natural sources. These compounds were subjected to their antiplatelet aggregation, antiinflammatory and antiallergic tests and the results were described as the following: l. Antiplatelet aggregation activity (1) Compounds 4 and 6 showed significant inhibitory effects on the platelet aggregation induced by collagen (Col.) with IC50=100 μg/ml and 40 μg/ml, respectively. (2) Compound 8 showed significant inhibitory effects on the platelet aggregation induced by arachidonic acid (AA) at the concentration of 100 μg/ml. 2. AntiinfIammatory and antiallergic activity (l) Compound 2l showed significant inhibition of release of b-glucuronidase with IC50=10.2 μM and lysozyme with IC50=11.6 μM from rat neutrophils induced by FMLP. (2) Compound 24 showed significant inhibition of release of b-glucuronidase with IC50=57.5 μM and lysozyme with IC50=29.0 μM from rat neutrophils induced by FMLP. (3) Compound 26 showed significant inhibition of release of b-glucuronidase with IC50=15.0 μM and lysozyme with IC50=29.0 μM from rat neutrophils induced by FMLP. (4) Compound 21, 24 and 26 inhibited superoxide formation induced by FMLP from rat neutrophils with IC50=10.0, 48.5 and 36.0 μM, respectivery. The bioactivity of those compounds are under proceeding.