Apoptosis in Rheumatoid Arthritis

• Main Authors: Yang,Jia-Sing, 楊家欣
• Other Authors: Lee,Miau-Rong
• Format: Others
• Language: zh-TW
• Published: 1998
• Online Access: http://ndltd.ncl.edu.tw/handle/80879094211990101224

碩士 === 中國醫藥學院 === 醫學研究所 === 86 === Rheumatoid arthritis (RA) is a chronic inflammatory disease. Synovialtissue f rom patients with RA is characterized by pronounced hyperplasia andinflux of a variety of inflammatory cells. Recent studies have considered that irregular apoptosis pathway maybe one of the pathogenic mechanism in RAsynovium. In thi s study, we found that the majority of apoptotic cells detectedby TUNEL method were fibroblast like synoviocytes and some infiltratingmononuclear cells in t he sub -lininglayers of the RA synovium tissue. By using single or double immu nohistochemical staining assays we comparedin situ expression of Fas, FasL, p5 3 and Bcl-2 in RA, OA and post-traumaticsynovial tissue. Our results demonstra ted significant expression Fas, FasL andp53 in RA synovial tissue compared wit h that in OA or post-traumatic synovialtissue. No significant difference of B cl-2 expression was detected betweenpatient groups. Bcl-2 expression was main ly detected in infiltratinglymphocytes clustered around theblood vessels in su b -lining layers. In RA synovial tissue, expression of Fas, p53 was detected in sub -lininglayer. The majority of Fas, p53 expressing cells were fibroblas t likesynoviocytes and a few infiltratingmononuclear cells. Coexpression of F as andp53 cells were also mainly detected in fibroblast like synoviocytes of s ub-lining layer. FasL expression was mainly detected in infiltrating mononucle arcells and a few fibroblast like synoviocytes in sublining layers. Occasional ly, FasL positive cells were detected around Fas positive cells. A few mononuc learcells coexpressed both Fas and FasL.In addition, we observed that in RA sy novial tissue, one third of Fas positivecells were TUNEL positive. Some p53 po sitive cells were also TUNEL positive. these resultsindicated that Fas or p53 mediated apoptosis may occur during RApathogenesis. Further studies are on pr ogress to examine the relationship ofp53 and Fas mediated apoptosis pathway in RA fibroblast like synoviocytes.We also analysis the p53 gene of patient's sy novium and peripheral blood cellsto investigate whether mutant p53 gene may co ntribute to the abnormal growth of RA synovium. By SSCP polymorphism and DNA sequencing of p53 gene exon 4 and 5, Our preliminary results indicates that no ne of the samples contain mutant type p53 gene.