| Summary: | 碩士 === 中國醫藥學院 === 醫學研究所 === 86 === Rheumatoid arthritis (RA) is a chronic inflammatory disease. Synovialtissue f
rom patients with RA is characterized by pronounced hyperplasia andinflux of a
variety of inflammatory cells. Recent studies have considered that irregular
apoptosis pathway maybe one of the pathogenic mechanism in RAsynovium. In thi
s study, we found that the majority of apoptotic cells detectedby TUNEL method
were fibroblast like synoviocytes and some infiltratingmononuclear cells in t
he sub -lininglayers of the RA synovium tissue. By using single or double immu
nohistochemical staining assays we comparedin situ expression of Fas, FasL, p5
3 and Bcl-2 in RA, OA and post-traumaticsynovial tissue. Our results demonstra
ted significant expression Fas, FasL andp53 in RA synovial tissue compared wit
h that in OA or post-traumatic synovialtissue. No significant difference of B
cl-2 expression was detected betweenpatient groups. Bcl-2 expression was main
ly detected in infiltratinglymphocytes clustered around theblood vessels in su
b -lining layers. In RA synovial tissue, expression of Fas, p53 was detected
in sub -lininglayer. The majority of Fas, p53 expressing cells were fibroblas
t likesynoviocytes and a few infiltratingmononuclear cells. Coexpression of F
as andp53 cells were also mainly detected in fibroblast like synoviocytes of s
ub-lining layer. FasL expression was mainly detected in infiltrating mononucle
arcells and a few fibroblast like synoviocytes in sublining layers. Occasional
ly, FasL positive cells were detected around Fas positive cells. A few mononuc
learcells coexpressed both Fas and FasL.In addition, we observed that in RA sy
novial tissue, one third of Fas positivecells were TUNEL positive. Some p53 po
sitive cells were also TUNEL positive. these resultsindicated that Fas or p53
mediated apoptosis may occur during RApathogenesis. Further studies are on pr
ogress to examine the relationship ofp53 and Fas mediated apoptosis pathway in
RA fibroblast like synoviocytes.We also analysis the p53 gene of patient's sy
novium and peripheral blood cellsto investigate whether mutant p53 gene may co
ntribute to the abnormal growth of RA synovium. By SSCP polymorphism and DNA
sequencing of p53 gene exon 4 and 5, Our preliminary results indicates that no
ne of the samples contain mutant type p53 gene.
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