| Summary: | 碩士 === 台北醫學院 === 醫學研究所 === 85 === The re-used deep-frying fats, 8 samples collected
arbitrarily again from the vendors' stands and restaurants
after sensory indication of abuse and 7 samples collected from
the laboratory by which 5 samples only fried each one kind of
food and 2 samples fried many kinds of food were used to
analyze any existence of genotoxicity. After ISO (
International Organization for standardization ) polar
separation of the oil samples, the Ames mutagenicity test was
applied to analyze their genotoxic activities. The tester
strains included Salmonella typhimurium TA98 and TA100 for
detecting the direct or indirect mutagenic activities.
According to the results of the precedent studies, the
contamination of primary mutagens was detected by using
the tester strain of base-pair substitution; however , the
major mutagenic activity came from the secondary mutagens by
frame-shift replacement. Therefore, the TA100 strain without S9
mix and TA98 strain with S9 mix were used to test again
the genotoxic effects of the deep-frying fats.
The results showed that there were no any mutagenic reaction
among re-useddeep-frying fats from the vendors'stands and
restaurants and the conditionallaboratory products when we only
fried one kind of food for a short term at hightemperature above
200℃. On the other hand, the most mutagenic activities
wereobviously measured out with dose-response from the non-
polar, polar and very-polar fractions of the continuously-used
oil when we fried many kinds of food for 30 hrs in the
laboratory,but when we fried them up to 40 hrs,the mutagenic
activities almost disappeared. At present, the re-used oils of
the vendors' stands and restaurants could be said
hygienically safe under the general cooking condition here in
Taiwan. Nevertheless,the re-used oil could further turn to be
genotoxic when they were seriously abused during such as a
long-term frying.
|
|---|
