| Summary: | 碩士 === 東吳大學 === 微生物學系 === 85 === Immunization with plasmid DNA encoding antigenic proteins
elicits both antibody and cell-mediated immune responses.DNA
immunization has provided effective protective immunity in
various animals models.The immune responses induced by DNA
vaccines may in some instances be preferable to those produced
by immunization using conventional methods.DNA vaccination
appears to be applicable to a variety of pathogens and is a
useful method of raising immune responses.Thus this approach to
vaccination has the potential to be a successful method of
rapidly screening for antigens capable ofinducing protective
immunity,and of inducing protective immunity against pathogens
of clinical importance.DNA vaccine is better than traditional
vaccinesin a serial of tests so far but the using of DNA vaccine
in human bodies should be more serious considered and tested.
Because the pig farming is the most important animal husbandry
in Taiwan,here we choose a virology disese of swine,swinw
vesicular disease(SVD),to develop the DNA vaccine using in them
and we can also establish a modle from this study to set up the
developing method of DNA vaccine.The structure protein,VP1,of
swine vesicular disease virus was thought to be an antigen that
can induce the protective antibody to swine so the VP1 gene was
chosen to develop DNA vaccine of swine vesicular disease.The
first step in developing of DNA vaccine of SVD is the expression
of VP1 gene in muscle cell,VP1 gene was insert an eukaryotic
vector,pcDNA3,an can express a 30 kDa protein in vitro in this
study.This Vp1 expression construct can also express VP1 mRNA in
C2C12 mouse muscle cell line and can be observed by using RT-PCR
technique.The result indicates that these VP1-pcDNA3 construct
could also express VP1 protein in muscle.The mouse immunized
with VP1-pcDNA3 may produce antibodies and after collecting the
serum of mouse,we use immunoprecipitation and western blotting
to examine the serum.The results infer that there are anti-VP1
antibodies produced.
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