| Summary: | 碩士 === 東吳大學 === 化學系 === 85 === The abuse of amphetamine and methylamphetamine is a serious
problem in Taiwan. The determination of amphetamine and
relatedcompounds in human urine sample is an important task.
Highperformance liquid chromatography (HPLC), gas chromatography
(GC)or gas chromatography/mass spectrometry (GC/MS) has been
widely used for the determination of amphetamine and related
compounds in biological fluids .The purpose of this study is to
evaluate the effectiveness of HPLC/ES/MS for the analysis of
methylamphetamine, amphetamine, ephedrine, and
phenylpropanolamine in human urine sample. HPLC/electrospray/
MS (HPLC/ES/MS) has emerged as an effective and useful
analytical technique. ES ionization produces ions at atmospheric
pressure and relatively low temperature which minimizes thermal
decomposition of labile compound. Furthermore, aqueous sample
can be analyzed with little or no sample preparation. In this
study, we developed a LC/ES/MS method for the detection of
amphetamine and related compounds. The effect of ES/MS capillary
exit (CapEx) volts, solvent composition, and modifier on the
detection were investigated. A simple but effective sample
preparation technique was developed for urine sample. It
utilized microfiltration membrane to remove proteins and other
compounds in urine prior to LC/ES/MS analysis. Good linear range
(100~1000 ng/ml) and low detection limit (10 ng/ml, S/N=3) were
achieve by SIM. Finally, this method will be applied to analyze
the urine samples from drug addicts.
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