Development of the Determination of Parabens by Supercritical Fluid Extraction and Capillary Electrophoresis

• Main Author: 張清莉
• Other Authors: 王書蘋
• Format: Others
• Language: zh-TW
• Published: 1997
• Online Access: http://ndltd.ncl.edu.tw/handle/36635095186725501354

碩士 === 靜宜大學 === 應用化學研究所 === 85 === Pararbens are important additives in cosmetics for inhibiting the development of micro-organism. However, all the preservatives are harmful to their consumers owing to their potency to induce contact dermatitis or allergic. Therefore, it is very important to assay these additives in cosmetics with high precision and good accuracy. This paper proposed a novel analytical method for determination of parbenes in cosmetic products was extracted by supercritical fluid extraction (SFE) and followed by analyzed with capillary zone electrophoresis (CZE) and high performance liquid chromatography (HPLC) for further analysis. The optimum operational condition for CZE to use was 30.0mM sodium tetraborate in 5% acetontrile as buffer solution, and the capillary was fused silica-75μm, 60cm, at 25kV, 30℃. The good resolution (Rs=1.70), linear range (350ppb-60.0ppm), calibration curves (correlation coefficient, R2=0.9991-0.9998, n=12) and detection limit (347-350ppb) were obtained for four parabens. In the HPLC determination, the satisfied resolution (Rs=1.63), lower and more narrow linear range (54.7ppb-8.00ppm), calibration curve (R2=0.9997-0.9999, n=10) were obtained for the four parabens by gradient elution. The detection limit for the four parabens was 7.08-17.2ppb. The optimum extraction condition of SFE was 0.05% acetonitrile-carbon dioxide as supercritical fluid at 40℃/1750 psi to extract parabens in cosmetics. Then followed to analysis by HPLC. The high recovely (94.5-101%) and good relative standard derivation (RSD=0.06-3.75%) were obtained. These excellent analytical techniques were applied to ten commercial cosmetics for direct determination of parabens. These cosmetics were extracted by SFE and followed analysis by HPLC and CZE. Parabens are the popular antiseptic in all cosmetics, especially for methyl parabens and propyl paraben. In this research, the confidence in qualitative analysis by identifying the authentic diagrams of spectroscopy and the purity indexs were acquired by spiked method also. And the recoveries were 99.8-105% and reproducibility 0.260-3.54% were obtained which process high precision and accuracy in the analytical method.