Effects of nucleoside-nucleotide mixture on memory and brain neurotransmitter in mice

• Main Authors: Wu, Hsueh-Hua, 吳雪華
• Other Authors: Wang Ming-Fu
• Format: Others
• Language: zh-TW
• Published: 1997
• Online Access: http://ndltd.ncl.edu.tw/handle/48779749208545596268

碩士 === 靜宜大學 === 食品營養學系 === 85 === Alzheimer''s disease is a continued and incapable of restoring brain neurodegenerative disease, which causes memory impairment. Extracellular nucleosidesand nucleotides can stimulate proliferation of astrocytes, nerve growth factor (NGF) synthesis, and increase the concentration of NGF in brain, which ameliorate age-related memory deficits in rodents. We studied the effect of dietary nucleosidesand nucleotides mixture (NNM) on memory deficient mice, and analyzed the brain neurotransmitter in mice. The experiment used 1 and 7 month old male senescenceaccelerated mice (SAM), 1 month old male ddY and poor learning mice (PLM). Theywere separated to two groups, one group was fed with 20% casein diet (control),the other fed with 20% casein diet supplemented with 0.5% NNM for 12 weeks. Theambulatory activity of the mice was measured after 3 weeks, and memory test wasstudied by a single-trial passive avoidance test after 4,8, and 12 weeks. After finishing behavioral test, mice were killed, each brain was dissected into the four brain regions (cortex, hippocampus, striatum and substantia nigra), tissues were homogenized, after centrifuged and filtrate was directly injected into the microbore column high performance liquid chromatography-electrochemical detection(microbore HPLC- ECD) system and the metabolites of monoamine neurotransmitterwere be assayed. The results show that body weight, food intake and locomotion of the mice areabout the same between the two groups. In those memory test, there are not significanted effect on 1 month old SAM-P/8 strain mice fed with supplemented 0.5% NNM. For the 7 month old SAM-P/8 strain mice fed with supplemented 0.5% NNMafter 12 weeks, we measured the average time of successful memory 24 hours after the punishment and we found it significantly higher than the control group (p<0.05).In the ddY strain mice during the development of early brain period, the diet withsupplemented 0.5% NNM that can enhance memory ability. Whereas the diet supplementedwith 0.5% NNM for 1 month old PLM mice after 8 and 12 weeks was better than thecontrol group. Moreover, the level of the monoamine and their metabolites increasein cortex, hippocampus, striatum and substantia nigra of the experimental mice,compared with the control group mice. To sum up, the present finding is supported by the dietary administration of 0.5% NNM, that improves in the ability of memory deficient mice, and decreases the decay of brain monoamine neurotransmitter and their metabolites. Furthermore,the relationship between memory improvement and the brain monoamine neurotransmitterwould need explored in the future.