The Significance of Neural Cell Adhesion Molecule in Metastatic Tumors in central Nervous System

• Main Authors: Wang, Yi-Chung, 王一中
• Other Authors: Kou Yang Ming
• Format: Others
• Language: zh-TW
• Published: 1997
• Online Access: http://ndltd.ncl.edu.tw/handle/65302045560861025297

碩士 === 文化大學 === 應用化學系 === 85 === The progress in medicine has made clinicians able to diagnose，treat and even cure many diseases，but cancer is still one of the leading causes of patient's mortality。Although the etiology of most of cancers are unclear，treatment of primary tumors gave significant success in recent years hence decreased the morbidity and prolonged the life of patients。 However，the metastatic cancer remains one of the most unfavorable condition for therapy。The mechanism of metastasis of malignant tumor cells from a primary site to near or distant secondary sites belong to the most important problems in carcinogenesis。 Many cancers display characteristic organ colonization patterns that do not fit simple，anatomical-mechanical trapping theories of tumor cell dissemination。Proposed underlying mechanisms of the organ preference of metastatic spread include the specific binding between tumor cells and endothelial cells in the target tissue。The specific interaction between tumor cells and extracellular matrix of the target tissue may also involve this process。 Neural cell adhesion molecule ( N-CAM ) belongs to the immunoglobulin gene superfamily。It has been studied thoroughly for decades，and demonstrated to be crucial in development of nervous system，neuroplasticity and regeneration 。In oncology research，the N-CAM has been suggested to be a useful surface marker and it's expression correlates closely with the prognosis of small cell of lung cancer ( SCLC )，and is likely involved in early CNS metastasis of SCLC。 There are two main purposes in this project，the first one is to provide basic clinical and pathological data among our Chinese population through an extensive study of the cases of CNS metastatic tumors from the database of VGH-Taipei；the second one is to investigate the involvement of N-CAM in tumor metastasis to central nervous system。The in vitro response of tumor cells to purified N-CAM molecules and monoclonal antibody ，the antibody- and complement- related lysis of tumor cells expressed N-CAM will be investigated in our laboratory in the further。The whole series of research will give us information about the potential preventive or therapeutic management of metastatic tumors in CNS。 In the first part of this project ，some of the major clinical and pathological features of the primary and CNS metastatic tumors will be analyzed and compared to the published data from other sources。 At the beginning of the second part of this project，monoclonal antibody 12F8 ( recognize N-CAM ) and some commercially available antibodies recognize N-CAM ( on different epitopes ) will be used to study the expression of N-CAM on various tumor cells from patients as well as from the cell bank in Veterans General Hospital-Taipei ( VGH-Taipei )。 In the further studies related to the current project，human N-CAM will be purified from fresh neurogenic tumor using immunoaffinitity column。Tumor cells with high level expression of N-CAM ( determined in this project by immunohistochemical stain ) will be maintained in culture。 Purified human N-CAM and specific binding antibodies on tumour cells will be evaluate in vitro system。 This project will be accomplished under the team work of clinician，pathologist and personnel skillful in basic research to tackle the problem related to tumors metastasize to central nervous system，will thus provide a panoramic view of this particular constellation of clinical disease，hopefully，provide possible managements to prevent the occurrence or to treat the metastasized tumors。