To Study the Effects of Sulfate on Glutathione Levels of Water Convolvulus by High Performance Liquid Chromatography

• Main Authors: Lee, Chin-Shyang, 李清祥
• Other Authors: H.H.Chen
• Format: Others
• Language: zh-TW
• Published: 1997
• Online Access: http://ndltd.ncl.edu.tw/handle/60458930037396319508

碩士 === 文化大學 === 應用化學系 === 85 === The objective of this thesis is to study the effects of pure water and 0.05M sodium sulfate on the glutathione levels of water convolvulus. The water convolvulus of 30 days ages was cultivated in pure water and 0.05M sodium sulfate solution at different times (0,24,48,72,and 96 hours)and determined the levels of glutathione in leaves, respectively. The results found that there is a correlation between the levels of glutathione in water convolvulus and the cultivated sodium sulfate concentration up to72 hours . The levels of glutathione in the leaves of 0.05M sodium sulfate treated water convolvulus at 72 hours and are approximately 1.5 times higher than the corresponding control value. The increases of glutathione dropped gradually thereafter,due to the effect of sodium sulfate on the growth deterioration of water convolvulus. The levels of glutathione of water convolvulus leaves which cultivated in pure water decrease as the times increase.The Glutathionelevels of water convolvulus in 0.02M sulfate solutions at different time intervals treated as 100%. At 0.1M sulfate solutions, the Glutathione levels from 24 to 72 hours increased 1.5 times; then drop to 1.2 times at 96 hours. Those results show that the rate of biosynthesis of Glutathione ishigher up to 72 hours if cultivated at 0.1M sulfate solution. Both species cultivated in pure water and the Glutathione levels of water convolvulus for different time intervals treated as 100%. The growing trends of corresponding Glutathione levels of 20 and 45 days old spinach drop gradually from 116% to 75.9% and from 230.2% to 198.6%,respectively as times increase. These results show that Glutathione levels of 20 and 45 days old spinach has approximately +-20% and +-10% wider ranges of depletion, respectively as compared with water convolvlus. Both species cultivated in 0.1M sulfate solution and the Glutathione levels of water convolvulus at different time intervals treated as 100%. The growing trend of corresponding glutathione of 20 days old spinach shows similarly as water convolvulus. The growing trend of Glutathione of 45 days old spinach decline gradually as times increasa. These results show that nature spinach may not need to increase the biosynthesis of Glutathione similarly as water convolvulus even cultivated at 0.1M sulfate solution. The leaf Glutathione levels were determined by high performance liquid chromatograpry.