| Summary: | 碩士 === 文化大學 === 應用化學系 === 85 === The objective of this thesis is to study the effects of
pure water and 0.05M sodium sulfate on the glutathione levels of
water convolvulus. The water convolvulus of 30 days ages was
cultivated in pure water and 0.05M sodium sulfate solution at
different times (0,24,48,72,and 96 hours)and determined the
levels of glutathione in leaves, respectively. The results found
that there is a correlation between the levels of glutathione in
water convolvulus and the cultivated sodium sulfate
concentration up to72 hours . The levels of glutathione in the
leaves of 0.05M sodium sulfate treated water convolvulus at 72
hours and are approximately 1.5 times higher than the
corresponding control value. The increases of glutathione
dropped gradually thereafter,due to the effect of sodium sulfate
on the growth deterioration of water convolvulus. The levels of
glutathione of water convolvulus leaves which cultivated in pure
water decrease as the times increase.The Glutathionelevels of
water convolvulus in 0.02M sulfate solutions at different time
intervals treated as 100%. At 0.1M sulfate solutions, the
Glutathione levels from 24 to 72 hours increased 1.5 times; then
drop to 1.2 times at 96 hours. Those results show that the rate
of biosynthesis of Glutathione ishigher up to 72 hours if
cultivated at 0.1M sulfate solution. Both species cultivated in
pure water and the Glutathione levels of water convolvulus for
different time intervals treated as 100%. The growing trends of
corresponding Glutathione levels of 20 and 45 days old spinach
drop gradually from 116% to 75.9% and from 230.2% to
198.6%,respectively as times increase. These results show that
Glutathione levels of 20 and 45 days old spinach has
approximately +-20% and +-10% wider ranges of depletion,
respectively as compared with water convolvlus. Both species
cultivated in 0.1M sulfate solution and the Glutathione levels
of water convolvulus at different time intervals treated as
100%. The growing trend of corresponding glutathione of 20 days
old spinach shows similarly as water convolvulus. The growing
trend of Glutathione of 45 days old spinach decline gradually as
times increasa. These results show that nature spinach may not
need to increase the biosynthesis of Glutathione similarly as
water convolvulus even cultivated at 0.1M sulfate solution. The
leaf Glutathione levels were determined by high performance
liquid chromatograpry.
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