| Summary: | 碩士 === 中國文化大學 === 生物科技研究所 === 85 === There are two mating types of the haploid Saccharomyces
cerevisiae alpha and a . An alpha haploid cell type secretes the
alpha mating factor and an a cell type secretes the a factor .
The alphais a short pheromonepeptie which contains 13 amino
acids . The first 6 amino-acid residues in the N-terminal
sequenceof the alpha factor are responsible for the
divisionarrest of the a-type S.cerevisiae . However the function
of the other 7 C-terminal amino acids of the alpha factor(
alpha7) is still unknow . Although the secretion pathway of the
alpha factor has been described by alot of previous studies .
little information is available on the sorting of the alpha
factor in trans golgi . To obtain more information on the
function of the alpha7 , we synthesized the nucleotides coding
for the alpha7 and ligated this gene cassette to a reporter
gene , the green fluorescent protein (GFP) . By detecting the
green fluorescence , the distribution of the GFPalpha7fusion
protein is monitored . On the basis of this stratagem , we were
ableto examine the hypothesis that the alpha7 play an important
role in sortingof the alpha factor from trans golgi to vesicles
and , thus , to secrete outside the cell . A signal peptide
in N-terminal of GFP was found to be required for the
introduction of the GFPalpha7 fusion protein into secretion
pathway .
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