| Summary: | 碩士 === 國立台灣工業技術學院 === 化學工程技術研究所 === 85 === In this study，IM60 (Lipase from Mucor miehei immobilized on
anionic resin) was employed to catalyze acidolysis between
borage oil and n-3 polyunsaturated fatty acid (PUFA) obtained
from the saponification of menhaden oil. The acidolysis between
mixtures of acylglycerols obtained from the partial hydrolysis
of borage oil (catalyzed by Candida rugosa lipase immobilized on
microporous polypropylene) and PUFA (derived from menhaden oil)
was also studied. The effects of temperature, enzyme loading,
substrate concentrations, and substrate weight ratio on the
reaction product were investigated. The purpose of this work is
to obtain acylglycerols rich in both n-3 and n-6 fatty acids.For
acidolysis catalyzed by IM60, with concentrations of borage oil
and n-3 PUFA equal to 20mg/ml and 60mg/ml, respectively, and a
reaction temperature of 50℃, the content of GLA, EPA and DHA in
acylglycerols can reach 20.6%, 19.1%, and 14%, respectively.When
borage oil was hydrolyzed for 4h before n-3 PUFA was added to
the acidolysis for another 18h, GLA, EPA and DHA content in
acylglycerols reach 26.5%, 19.8% and 18.1%, respectively.The
hydrolysis of menhaden oil (catalyzed by immobilized Candida
rugosa lipase) yields acylglycerols with EPA and DHA content of
19.4% and 25.6%, respectively, after a reaction time of 8h. The
acidolysis of this acylglycerol and n-3 PUFA derived from
saponification of borage oil (with weight ratio of 1:1) was
catalyzed by IM60 for 12h. GLA, EPA and DHA content in
acylglycerols in the product are 16.7%, 12.8% and 19.2%,
espectively.
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