Functional Analysis of α-Amylase Genes in Rice Using Antisense Approach
碩士 === 國立臺灣大學 === 農藝學研究所 === 85 === Expression of α-amylase gene in O.sativa (rice) suspension cultured cell is induced by sucrose starvation. Accumulation of all the α-amylase mRNA increased in response to sucrose starvation, mRNA abundance of aAmy3 was distinctly higher than other α-amylase g...
| Main Authors: | , |
|---|---|
| Other Authors: | |
| Format: | Others |
| Language: | zh-TW |
| Published: |
1997
|
| Online Access: | http://ndltd.ncl.edu.tw/handle/50111113288585294408 |
| Summary: | 碩士 === 國立臺灣大學 === 農藝學研究所 === 85 ===
Expression of α-amylase gene in O.sativa (rice) suspension cultured cell is induced by sucrose starvation. Accumulation of all the α-amylase mRNA increased in response to sucrose starvation, mRNA abundance of aAmy3 was distinctly higher than other α-amylase gene and played a important role in seed germination. To study the effects of sugar m tabolite regulation on the individual α-amylase gene, cells were transformed by particle bombardment, with plasmid carrying the 3' untranslated region (3' UTR) of αAmy3 (αAmy3D). In the antisense αAmy3 3'UTR sequence containing cell line, significant decrease in the level of αAmy3 transcript and total α-amylase content were observed on sucrose-free induction promoter of α-amylase. This thesis also demonstrate that it is a possible to produce and maintain a period of time of transgenic rice suspension cell lines from immature embryo.
|
|---|