The Bioactive Constituents of Drypetes hieranensis

• Main Authors: Chen, Chia-Hua, 陳家華
• Other Authors: Shoei-Sheng Lee, Karin C. Liu
• Format: Others
• Language: zh-TW
• Published: 1997
• Online Access: http://ndltd.ncl.edu.tw/handle/89591151754309317550

碩士 === 國立臺灣大學 === 藥學系 === 85 === The Bioactive Constituents of Drypetes hieranensis In the course of biological activity screening，the ethanolic extract of the leaves of Drypetes hieranensis Hay.( Euphobiaceous )，exhibited inhibitory effect against EBV- DNA polymerase．Hence, this study was aimed to study the boactive ingredients of this plant. The ethanolic extract of the leaves was divided into fractions soluble in chloroform, ethyl acetate， n-butanol and H2O by liquid-liquid partitioning. The chloroform soluble fraction was repeatedly chromatographed on silica gel columns to give compounds ( 12 ) ~ (17 ) and ( 9 )．Separation of the ethyl acetate and n-butanol soluble fractions over Sephadex LH-20 ，centrifugal partition chromatography ( C.P.C. )，and Toyoperal HW-40C columns afforded compounds ( 1 ) ~ ( 8 ) and ( 10 )~( 11 )．The structural elucidation of these compounds was conducted mainly by spectroscopic analysis. According to properties of the structures, they were classified into five categories: 1. Biflavones: podocarpusflavone A ( 1 ), amentoflavone ( 2 ) 2. Polyphenolic compounds: ethyl brevifolin carboxylate ( 3 ), ethyl gallate ( 4 ) ellagic acid ( 5 ), ellagic acid 4-O-α-L-rhamnoside ( 6 ), ellagic acid 4-O-β-D-xyloside ( 7 ), 3,3-O,O- dimethylellagic acid 4-O-α-L- rhamnoside ( 8 ), hexamethylcoruleoellagic acid ( 9 ). 3. Tannins: corilagin ( 10 ), geraniin ( 11 ) 4. Sterols: β-sitosterol / stigmasterol ( 12 ), β-sitosterol 3-β-D- glucoside / stigmasterol 3-β-D-glucoside ( 13 ). 5. Triterpenes: squalene (14), 3-oxofriefelan-30-ol (15), 3β,30- dihydroxyfriedelane (16) Among compounds isolated from ethyl acetate and n-butanol， componds (2) 、( 5 )、( 10 ) and (11) show anti-HIV-1 reverse transcriptase activity with the IC50 of 17.6, 21.9, 33.8 and 17.5μM respectively；besides，compounds (10)and ( 11 ) also showed anti-EBV DNA polymerase activity at IC50 29.0 and 27.1 μ M respectively．