| Summary: | 碩士 === 國立臺灣大學 === 藥學系 === 85 === This thesis is composed of two parts:Part I : 1. Analysis of
Bisbenzylisoquinoline Alkaloids by High Performance Liquid
Chromatography2. Capillary Electrophoretic Separation of
Bisbenzylisoquinoline AlkaloidsPart II : Analysis of
Fluoroquinolone Antibacterials by Capillary ElectrophoresisPart
I1. Analysis of Bisbenzylisoquinoline Alkaloids by High
Performance Liquid ChromatographyA well-resolved high-
performance liquid chromatographic method has been developed for
the analysis of nine type VI and type VIII bisbenzylisoquinoline
alkaloids ( aromoline, obamegine, berbamine, homoaromoline,
colorflammine, thalrugosine, norobaberine, tetrandrine and
isotetrandrine. The method used the reversed-phase ion-pair
chromatography aided by the photodiode array UV detection. The
mobile phase was composed of 27 % volume of acetonitrile and 73
% volume of 0.05 M dihydogen phosphate buffer (pH 3.0) to which
0.1% diethylamine (v/v) and 2 mM sodium heptanesulfonate were
added. The method was applied to the analysis of these alkaloids
in the stem woods of a Lauraceous plant Dehaasia triandra Merr.
Five such alkaloids were identified and their contents were
determined. This method should be applicable to the other
Lauraceous plants and the various plants containing type VI and
type VIII bisbenzylisoquinoline alkaloids.2. Capillary
Electrophoretic Separation of Bisbenzylisoquinoline AlkaloidsIn
this study, the use of the overlapping resolution mapping scheme
to achieve an optimum capillary electrophoretic separation of
eight bisbenzylisoquinoline alkaloids, aromoline, obamegine,
berbamine, homoaromoline, colorflammine, thalrugosine,
tetrandrine and isotetrandrine was investigated. Three critical
parameters of electrophoretic media surfactant (sodium
cholate), organic modifier (acetonitrile) and pH were chosen to
carry out the process. Eight bisbenzylisoquinoline alkaloids
were baseline-separated within 20 minutes using the predicted
conditions of an optimum separation: buffer containing 132 mM
sodium cholate, 25.8% acetonitrile (v/v) and pH 7.58.Part
IIAnalysis of Fluoroquinolone Antibacterials by Capillary
ElectrophoresisTo meet the requirement of quantitative analysis,
this work re-investigated the parameters which were relevant to
the separation of the seven fluoroquinolone antibacterials,
sparfloxacin, lomefloxacin, norfloxacin, ciprofloxacin,
enoxacin, ofloxacin and pefloxacin, based on the method
developed previously in this laboratory. With the optimum
conditions obtained as follows: electrophoretic media ( 65 mM
sodium borate, 35 mM sodium dihydrogen phosphate, 60 mM sodium
cholate, pH 7.3 / ACN (72 : 28, v/v); fused silica capillary (
50 (m I.D., total length 67 cm, effective length 52 cm;
injection pressure ( 60 mbar, injection time ( 3 sec; voltage (
27 kV; detection wavelength ( 275 nm and temperature ( 25℃, the
fluoroquinolones were well separated within nine miniutes. After
validation with the reproducibility, linearity and accuracy
criteria the method was used for the assay of the commercial
formulation products. The assay results were within 95~105 % of
their labeled claims. The method should be applicable to the
analysis of these and other similar formulation products
containing these drugs.
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