| Summary: | 碩士 === 國立臺灣大學 === 獸醫學系研究所 === 85 === Knowlege of the gastrointestinal microflora is a fundation in understanding pa
thophysiology of gastrointestinal diseases. This is also important to the conc
ern of public health among pets and owners.Fifteen clinically normal dogs ( no
rmal group ) and six dogs with gastrointestinal clinical signs ( diseased grou
p ) were sampled for the gastrointestinal microflora investigation in the pres
ent study. Mouth and the rectum samples were taken by steriled swabs. Esophagu
s , stomach and the duodenum samples were taken via endoscopic forceps.The bac
teria isolated from the mouth of the 15 clinically normal dogs were Pasteurell
a spp, Staphylococcus spp, Neisseria spp, Candida spp, Corynebacterium spp, Es
cherichia spp and others; the bacteria isolated from the esophagus od these do
gs were PAsteurella spp, Agrobacterium spp, Enterobacter spp, Escherichia spp
and others; Pasteurella spp, Staphylococcus spp, Streptococcus spp and Acranob
acterium spp were the major bacteria colonised on the gastric mucosa of these
15 dogs; Acinetobacter spp, Bacteroides sppp, Candida spp,
Listeria spp, Pasteurel
la spp and Streptococcus spp were the predominant bacteria isolated from the d
uodenum mucosa. Whereas Escherichia spp, Candida spp, Staphylococcus spp, Ente
robacterium spp, Proteus spp, Streptococcus spp, Corynebacterium spp, Enteroco
ccus spp and Klebsiella spp were the predominant bacteria isolated from the re
ctum of these 15 dogs. Most of the bacteria recovered from agar plates were th
e resident flora of the gastrointestinal tract in dogs. Campylobacter spp isol
atefrom the rectum of 2 normal dogs could be a potentially zoonotic pathogen w
hich might cause acute enteritis in human and young puppies. Bordetella bronch
iseptica, a respiratory pathogen in dogs, has been found on the gastric mucosa
, duodenum and the rectum from 2 clinically normal dogs with history of respir
atory problems. The major bacteria is lated from the mouth of 6 diseased dogs
were Staphylococcus spp, Candida spp, Enterobacter spp, Klebsiella spp and Pas
teurella spp. Whereas Escherichia spp, Proteus spp, Candida spp, Streptococcus
spp and Enterococcus spp were the predominant bacteria found in the rectum of
these dogs. To approach to the duodenum efficiently using gastroendoscope is
the major problem in the present study. Only 6 ( 6/16 ) dogs could be reached
to the duodenum for sampling. Administration of metoclopramide together with l
eft recumbency did not improve the procedure in the present study.
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