Isolation and Characterization of Digalactosyldiacylglycerols from Liagora orientalis J. Agardh and L. boergesnii Yamada, and Acylphloroglucinol from Zonaria diesingiana

• Main Authors: LIN, CHING-YU, 林靖愉
• Other Authors: CHOU HONG-NONG
• Format: Others
• Language: zh-TW
• Published: 1997
• Online Access: http://ndltd.ncl.edu.tw/handle/55329193977169290325

碩士 === 國立臺灣大學 === 漁業科學研究所 === 85 === Part I：Isolation and Characterization of Digalactosyldiacylglycerols from Liagora orientalis J. Agardh and L. boergesnii Yamada. Digalactosyldiacylglycerols resolved in a fraction was isolated from the acetone extract of cultured L. orientalis J. Agardh and L. boergesnii Yamada (Rhodophyta) filamentous phase via silica gel flash column chromotography. Structures of these compounds were identify as diglycosyldiacylglycerols by EIMS, FABMS, NMR and IR spectroscopic studies. Composition of the fatty acid residues of these diglycosyl-diacylglycerols from L. orientalis J. Agardh have been determined as C16:0 (58.57 %), C18:1 (16.48 ), C:5 (11.44 %), C20:4 (3.71 ), and C16:0 (58.57 %), C18:1 (16.48 ), C20:5 (11.44 ), C20:4 (3.71 %) from L. boergesnii Yamada by GC anaysis on their methyl esters. The sugar residues have been determined as 1, 6- linked digalactose through GC-MS analysis on their TMS- sugar derivatives, alditol acetates and partially methylated alditol acetates. The hemolytic and antiplatelet acativties of these digalactosyl- diacylglycerols were not observable. Part II：Isolation and Characterization of Acylphloroglucinols from Zonaria diesingiana J. Ag. Two acylphloroglucinols were isolated from Zonaria diesingiana J. Ag., a common species found along the northern coast in Taiwan. Based on 1H, 13C, 1H-1H COSY and 13C-1H COSY NMR data, one of the compounds, 2-(17'-hydroxy-1'-oxoeicosa-5',8',11',14' (all Z)-tetraenyl)-1,3,5-trihydroxybenzene, was determined and its 13C-NMR chemical shifts of C-3', C-4', C-16', C-18' were found different from the published data in Gerwick & Fenical (1982). The other one, 2-(1'-oxoeicosa-5',8',11',14', 17' (all Z)- pentaenyl 1,3,5-trihydroxybenzene, was also found to be different in its C-3' and C-4' 13C-NMR assignment with the data reported by Amico et al. (1981). This is the first report of these two compounds with their two-dimention NMR data. Broden peaks in the 1H-NMR spectrum showed the effects of intramolecular H-bonding in the spectrum of 2-(1'-oxoeicosa-5',8',11',14', 17' (all Z)- pentaenyl)- 1,3,5-trihydroxybenzene.