| Summary: | 碩士 === 國立臺灣大學 === 漁業科學研究所 === 85 === Part I：Isolation and Characterization of
Digalactosyldiacylglycerols from Liagora orientalis J. Agardh
and L. boergesnii Yamada.
Digalactosyldiacylglycerols resolved in a fraction was isolated
from the acetone extract of cultured L. orientalis J. Agardh and
L. boergesnii Yamada (Rhodophyta) filamentous phase via silica
gel flash column chromotography. Structures of these compounds
were identify as diglycosyldiacylglycerols by EIMS, FABMS, NMR
and IR spectroscopic studies. Composition of the fatty acid
residues of these diglycosyl-diacylglycerols from L. orientalis
J. Agardh have been determined as C16:0 (58.57 %), C18:1 (16.48
), C:5 (11.44 %), C20:4 (3.71 ), and C16:0 (58.57 %), C18:1
(16.48 ), C20:5 (11.44 ), C20:4 (3.71 %) from L. boergesnii
Yamada by GC anaysis on their methyl esters. The sugar residues
have been determined as 1, 6- linked digalactose through GC-MS
analysis on their TMS- sugar derivatives, alditol acetates and
partially methylated alditol acetates.
The hemolytic and antiplatelet acativties of these digalactosyl-
diacylglycerols were not observable.
Part II：Isolation and Characterization of Acylphloroglucinols
from Zonaria diesingiana J. Ag.
Two acylphloroglucinols were isolated from Zonaria diesingiana
J. Ag., a common species found along the northern coast in
Taiwan. Based on 1H, 13C, 1H-1H COSY and 13C-1H COSY NMR data,
one of the compounds, 2-(17'-hydroxy-1'-oxoeicosa-5',8',11',14'
(all Z)-tetraenyl)-1,3,5-trihydroxybenzene, was determined and
its 13C-NMR chemical shifts of C-3', C-4', C-16', C-18' were
found different from the published data in Gerwick & Fenical
(1982). The other one, 2-(1'-oxoeicosa-5',8',11',14', 17' (all
Z)- pentaenyl 1,3,5-trihydroxybenzene, was also found to be
different in its C-3' and C-4' 13C-NMR assignment with the data
reported by Amico et al. (1981). This is the first report of
these two compounds with their two-dimention NMR data. Broden
peaks in the 1H-NMR spectrum showed the effects of
intramolecular H-bonding in the spectrum of
2-(1'-oxoeicosa-5',8',11',14', 17' (all Z)- pentaenyl)-
1,3,5-trihydroxybenzene.
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