Variations of amylose and storage proteins in Tainan-White maize populations collected from six areas in Taiwan.

• Main Authors: Yeh, Chi-Shing, 葉志新
• Other Authors: Yih-Ching Huang
• Format: Others
• Language: zh-TW
• Published: 1997
• Online Access: http://ndltd.ncl.edu.tw/handle/45378041271901772281

碩士 === 國立臺灣大學 === 農藝學系 === 85 === Although Tainan-White maize has cultured for almost one hundred years in Taiwan, there is few understanding about this open pollinated population, especially qualitative traits such as the variation of storage proteins and amylose. The focus of this thesis was to determine the variations in content and molecular characteristics of storage protein and amylose in Tainan-White maize population collected from six areas in Taiwan, and to analysis the changes of storage proteins under different nitrogen treatments. Results are summarized as follows:1. After a long culture period at different areas, concentration and content of amylose and four storage proteins (albumins, globulins, glutelins and zeins) in six Tainan-white maize populations (Taipei, Tenchung, Tainan, Pingtung, Hwalian, Taitung) were significantly different. It suggests that the genetic components of Tainan-White maize population may already changed with respect to cultivation regions. 2. Z1 fraction of zeins of six Tainan-White maize populations were analyzed by RP- HPLC, 15-20 polypeptide peaks could be found in a single chromatographic profile, and in total 30 unique peaks were detected from the profile of all populations. In Z2 fractions, 2-3 major peaks and several minor peaks were identified. Some of the peaks appeared similar among regional populations. The results showed that zeins of six populations were different mainly in quantity but not in quality. Taipei population and Hwalian population had the largest polypeptide peaks appearing differently between each other. 3. The CV value of albumins, globulins, glutelins and zeins were 35.5%, 21.6%, 11.6% and 14.9, respectively. The CV value of the kernel weight was 17.7% and CV value of amylose was only 6%. Pintong population had the largest variability in kernel weight, concentrations of zeins, Z1 and globulins. All population had large variation in non- zeins protein fractions. There was a significant positive correlation between albumins and globulins, and between zeins and glutelins. The results imply that non-zein proteins have the potential to be improved by breeding selection.4. In the experiment of nitrogen treatments significat difference for investigated traits were found between Taipei and Hwalian populations. The amount of total storage proteins, zeins, Z1 and glutelins were also significantly different among treatments. There were no interaction between nitrogen fertilizer rates and populations. Increase of nitrogen rate would increase the concentration of zeins and glutelins. Concentration of zeins were increased majorly by the increase of 19kD and 22kD of Z1, and there were no change in Z2. The ratio of Z1/Z2 was increased under high nitrogen rates. In addition, albumins and globulins were not significantly different among three nitrogen fertilizer application rates.5. In nitrogen treatments, concentration of storage proteins in Taipei population were higher than that in Hwalian population. The responses of nitrogen, zeins and Z1 were different between two populations. Concentrations of zeins and Z1 in Taipei population increased to a maximum when the applied nitrogen rate reached 150 kg/ha, but concentration of zeins and Z1 in Hwalian population reached their maximum until nitrogen rate reached 300 kg/ha.