| Summary: | 碩士 === 國立臺灣大學 === 農業化學系 === 85 === Luminescence is a generic term that covers a range of process
which produce light. This study is restricted to the analytical
application of chemiluminescence arising during the course of a
chemical reaction, and bioluminescence a special type of
chemiluminescence found in biological system in which a
catalytic protein increase the efficiency of the
chemiluminescence reaction. Light is produced when molecules
reached to an excited state, and decayed to the ground state. We
try to use luminous bacteria to create a novel biosensing system
: Bioluminescent biosensing system in our laboratory. The study
can be separated several parts : the construction of
bioluminescent biosensing system, the confirm of the expression
vector and host cell; the physiological condition test of the
optimal host cell; the optimal test condition of the
bioluminescent biosensing system; the actual test of the toxic
compound. Our research construct expression vector (pUC-luxAB)
containing luciferase gene, and transformed into E. coli BL21
cell. The optimal incubation condition was : TB broth, 27℃,
18hrs. The bioluminescent biosensing system test condition was :
test temperature 35℃, using 100mL bacterial solution and 60mL
injection substrate's volume (10% n-Decyl aldehyde was soluble
in 9.5% ethanol) to produce the best bioluminescence. We also
select toxic molecules such as formaldehyde and EPN to detect
thesis toxicity in our experiment. According to experiment data,
it shows there is a linear correlation between the inhibition
rate of bioluminescence and concentration of toxic molecules :
formaldehyde and EPN, when their concentration range from 100 to
700ppm, 6.5 to 9.5ppm respectively.
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