| Summary: | 碩士 === 國立臺灣大學 === 園藝學系研究所 === 85 === Two types of fruit riping specific antisense ACC synthase gene, one with 180b
p invert repeat, pBI121-1ACC(+) and pBI121-aACC(-), and the other one without
the invert repeat, pBI121-aACC 1(-) were constructed. Transformants of banana
were efficiently produced from embryogenic suspension cells cocultivated with
Agrobacterium tumefaciens strain LBA4404 haboring b-glucuronidase, neomycin ph
osphate transferase II and antisense ACC synthase genes. The transformation ef
ficiency was affected by the cultivar of banana, the size of cell cluster, con
centration of Agrobacterium, light condition during cocultivation, subculture
time of suspension cells, and culture medium. The transformation efficiency pe
r gram suspension cells for Pei-Chiao-pBI121-aACC(-) was 22 transgenic cell li
nes, for Pei-Chiao-pBI121-aACC(+) was 77 transgenic cell lines, for Robusta-pB
I121 was 64 transgenic plants, for Robusta-pBI121-aACC(-) was 22 transgenic pl
ants. Most transformants showed normal phenotype and no
chimeric patterns was found. DNA analysis by PCR of the
transformants confirmed the integration of the foreign DNA.
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