The effects of water stress on the growth and membrane function of soybean plants (Glycine max L.)

• Main Authors: Liao, Ying-chao, 廖英超
• Other Authors: Huang Chi-ying
• Format: Others
• Language: zh-TW
• Published: 1997
• Online Access: http://ndltd.ncl.edu.tw/handle/89204980610631352654

碩士 === 國立臺灣大學 === 植物學系 === 85 === 8-day-old soybean seedlings were grown in hydroponics and in vermiculite system. Soybean which grow in hydroponics system was dried in air for 12 hours. Soybean which grow in vermiculite was treated with withholding water for 1, 2 and 3 days. After treatment, roots and leaves were harvested and water potential, fresh weight and dry weight were measured.Plasma membrane isolated from roots and leaves were purified by using two-phase partition method, and then assayed their ATPase activity, components of lipids and acyl side chains of lipids. The ATPase activity of vermiculite-growing soybean plants were higher than that grown in hydroponics. Water stress reduced the ATPase activity ofroots both in vermiculite-growing and hydroponics- growing plants. The ATPase activity of leaf was not reduced in hydroponics-growing soybeans after water-stress treatment. The ATPase activity was reduced after withholding water 2 days in vermiculite-growing soybean seedlings.The lipid quantity of plasma membrane was reduced by water-stress. Lipid composition of plasma membrane were changed. The lipids contained less unsaturated fatty acyl chains after water-stress treatment.