| Summary: | 碩士 === 國立臺灣大學 === 病理學研究所 === 85 === Solid tumor cytogenetic study had been quite underdeveloped compared with l
eukemia and lymphoma. There were many factors,including difficulties in tumor
culture and getting metaphase, and complex chromosome patterns. The rapid adva
nce in molecular cytogenetics, including fluorescence in situ hybridization an
d comparative genomic hybridization, has make great breakthoughin the study of
the solid tumor cytogenetic. My thesis included studies ina series of solid t
umors by using FISH technique. The first and second study allused chromosome p
ainting probes to identify the chromosome component of the marker chromosomes
in pulmonary hamartoma and dermatofibrosarcoma protuberans respectively.The th
ird study was chromosome aberrations in prostate cancer. We use Mega-YACprobe
for specific chromosome sites to study the deletion patterns. Our results reve
aled that chromosome 8p deletion is associated with the development of prostat
e cancer. The fourth study was chromosome aberrations in hepatocellular carcin
oma.We used 8 mega-YAC probes and 6 centromere probes on 17 hepatoma and one h
epatic adenoma. The results revealed chromosome 4q as the most frequent deleti
on site (76.5%),Chromosome 8p as the second most frequent site (59%). These t
wo chromosomesites should be the most important site harboring tumor suppresso
r genes related to the tumorigenesis of hepatoma.
|
|---|
