| Summary: | 碩士 === 國立臺灣大學 === 畜產學系 === 85 === This study aimed at the genetic polymorphism of swine
leucocyte antigen(SLA) class I and class II region in the Lee-
Sung strain of small-ear miniaturepig for researching about the
SLA genotype distribution in this population andsupporting a
good animal model in transplantation immunology research.
Bloodsample collects from 84 Lee-Sung strain of small-ear
miniature pigs, 5 small-earminiature pigs, and one male and one
female Landrace. Also, there are 47 DNAsamples of small-ear
miniature pig from animal germplasm conservation .
Afterisolating DNA from buffy coat with isolation kit, using
optimal primer to runpolymerase chain reaction (PCR) to
obtaining the target product, and checkingit with few product.
Others divide to equal part to restriction fragment
lengthpolymorphism (RFLP) analysis. The SLA-DQB region digests
by restriction enzymeHae III and Rsa I and SLA-DRB region by Msp
I and Rsa I. The result shows that :(1) After using the primer 1
and 5, the PCR product in the SLA class I region isabout 210bp,
but not good in specificity and repetition within this
population.(2) In the SLA class II region, use the primer SgDQB5
a/SgDQB3a, SgDQB5a/SgDQB3bin SLA-DQB loci and the primer SgDRB5
a/ SgDRB3a, SgDRB5a/SgDRB3b in SLA-DRB loci, the average PCR
product length are about 280bp, 270bp in SLA-DQB loci andabout
265bp, 245bp in SLA-DRB loci. After RFLP analysis, the SLA-DQB
loci isputative HA type by Hae III digested and RA type by Rsa I
digested. They arepossible recombination of e, g type by Hae III
digested and c, g mixed type byRsa I digested, compared to the
literature of Shia (1995). Also in the SLA-DRBloci, the genotype
is putative MA type by Msp I digested and RA type by Rsa
Idigested. In comparison with the literature of Shia (1995),
they are possible atype by Msp I digested and b type by Rsa I
digested. But in the DNA sample ofsmall-ear miniature pig from
animal germplasm conservation, the SLA-DQB PCR-RFLPpatterns have
three putative type by Hae III digested (HA, HB, and HC) and
fourputative type by Rsa I digested (RA, RB, RC, and RD). In
comparison with theliterature of Shia (1995), they are possible
recombination of e, g type (HA), d,e mixed type (HB) and e, g
mixed type (HC) by Hae III digested and c, g mixedtype (RA), a,
c, g mixed type (RB), c type (RC) and g type (RD) by Rsa
Idigested. This result is not consistent with the Lee-Sung
strain of small-earminiature pig, means that there if no genetic
polymorphism of SLA region in thispopulation after long-term
inbred breeding, but the real serological type andfull sequence
need further to be studied.
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