Antimutagenicity of water-soluble substance of adlay testa and its isolation and purification

• Main Authors: Tsai, Chih-Hung, 蔡至宏
• Other Authors: Wenchang Chiang
• Format: Others
• Language: zh-TW
• Published: 1997
• Online Access: http://ndltd.ncl.edu.tw/handle/24265932305041944263

碩士 === 國立臺灣大學 === 食品科技研究所 === 85 === During antimutagenicity study of adlay, our laboratory found that boilingwater extract of adlay testa(AT) had strong antimutagenic effect on IQ, butthere wa s still no any report on its mechanism and relating component. Themain purpose of this study was using AT as raw material to investigateantimutagenic effect of the boiling water extract of AT and its optimalextraction condition, and t ry to isolate and purify antimutagenic component.The result showed that (1)No mutagenicity or toxicity in Salmonellatyphimurium TA98 was observed with the b oiling water extract of AT;(2)according to the result of RSM design, antimutag enic effect of the boilingwater extract of AT was increased with increasing bo iling time and the ratioof water to material; (3)in Salmonella typhimurium TA 98 system, the extractof AT had no antimutagenic effect on NQNO, but had stron g effect on IQ, whichbelonged to desmutagenic, but not bio-antimutagenic; (4)a fter the extract wasfractionated into four fractions by membrane filters, the fraction which MWwas more than 3,000 had higher antimutagenic effect; (5)solub le dietary fiberobtained from the extract of AT had much stronger antimutegeni c effectsespecially the fraction which MW was between 30,000 and 100,000; (6)t heextract of AT also had good antioxidant activity and scavenging effects onsu peroxide anion and DPPH free radical.