Studies on the Reactions of Sulfamethazine and Sodium Nitrite

• Main Authors: Hung, Lan-hsin, 洪蘭心
• Other Authors: Hsu Shun-Yao
• Format: Others
• Language: zh-TW
• Published: 1997
• Online Access: http://ndltd.ncl.edu.tw/handle/18612603975159112366

碩士 === 國立臺灣大學 === 食品科技研究所 === 85 === Sulfamethazine is a commonly used animal drug. Its changes during processi ng and cooking and its degradation products are important subjects for studyin g. The purpose of this study is to investigate the effects of nitrite on sulfa methazine residue in pork muscle tissues. The study is divided into three part s. The in vitro conditions for the reactions between sodium nitrite and sulfam ethazine to take place was investigated in the first part. The reactions betwe en spiked sulfamethazine and sodium nitrite in pork muscle tissues was studied in the second part. The same reactions in pork muscle tissue incurred with su lfamethazine was investigated in the third part. The results from the first part in vitro study indicated that sodium nitrite and sulfamethazine reacted under acid conditions. The composition of its reaction products varied with p H values. Three reaction products with molecular weights of 503, 556 and 567 daltons, respectively, were detected in the reaction precipitate with a mass s pectrometry. The compound with molecular weight of 567 daltons could be 1,3-di -(4-[N-(4,6-dimethyl-2-pyrimidinyl)]sulphamoyl-phenyl)triazene. Three compound s with molecular weights of 307, 279 and 263 daltons, respectively, were detec ted with a HPLC at retention times of 6, 19 and 37 minutes, and could be 4-dia zohydroxy-N-(4,6-dimethyl-2-pyrimidinyl)benzenesulfonamide、4-hydroxy-N-(4,6-d imethyl-2-pyrimidinyl)benzenesulfonamide and desaminosulfamethazine, respectiv ely. The results from the second part of the study indicated that spiked su lfamethazine can react with sodium nitrite ( pH 1.4 ) to produce the compounds of molecular weights of 279 and 263 daltons in the presence of pork muscle ti ssue. The result from the third part of the study indicated that only one majo r reaction product was detected with LC-MS spectrometry when sulfamethazine wa s incurred in pork tissue. Its molecular weight is 263 daltons and it could be desaminosulfamethazine.